Characterization of a native hammerhead ribozyme derived from schistosomes
نویسندگان
چکیده
منابع مشابه
Characterization of a native hammerhead ribozyme derived from schistosomes.
A recent re-examination of the role of the helices surrounding the conserved core of the hammerhead ribozyme has identified putative loop-loop interactions between stems I and II in native hammerhead sequences. These extended hammerhead sequences are more active at low concentrations of divalent cations than are minimal hammerheads. The loop-loop interactions are proposed to stabilize a more ac...
متن کاملThe hammerhead ribozyme
The hammerhead ribozyme is a small RNA motif consisting of three helices that intersect at a conserved core. When correctly folded, the hammerhead ribozyme stimulates nearly complete cleavage of the phosphodiester chain at a defined internal site to give 2',3'-cyclic and 5'-hydroxy termini. The cleavage rate is approx. 1 min-' at 25 "C, pH 7.5, and increases proportionally with hydroxide ion co...
متن کاملThe ubiquitous hammerhead ribozyme.
The hammerhead ribozyme is a small catalytic RNA motif capable of endonucleolytic (self-) cleavage. It is composed of a catalytic core of conserved nucleotides flanked by three helices, two of which form essential tertiary interactions for fast self-scission under physiological conditions. Originally discovered in subviral plant pathogens, its presence in several eukaryotic genomes has been rep...
متن کاملThe unforeseeable hammerhead ribozyme
Despite its small size, the complex behavior of the hammerhead ribozyme keeps surprising us, even more than 20 years after its discovery. Here, we summarize recent developments in the field, in particular the discovery of the first split hammerhead ribozyme.
متن کاملHammerhead ribozyme kinetics.
The hammerhead ribozyme is a small RNA motif that self cleaves at a specific phosphodiester bond to produce 29,39 cyclic phosphate and 59 hydroxyl termini (Hutchins et al+, 1986; Forster & Symons, 1987a)+ The secondary structure of the hammerhead consists of three helices of arbitrary sequence and length (designated I, II, and III) that intersect at 15 nucleotides termed the catalytic core (Fig...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: RNA
سال: 2005
ISSN: 1355-8382
DOI: 10.1261/rna.7950605